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Image Search Results
Journal: Cancer Immunology Research
Article Title: T Cells Expressing Receptor Recombination/Revision Machinery Are Detected in the Tumor Microenvironment and Expanded in Genomically Over-unstable Models
doi: 10.1158/2326-6066.cir-20-0645
Figure Lengend Snippet: Figure 2. T cells expressing Rag1/2 and Dntt increase in Mlh1-ko 4T1 tumors. A–H, Representative input (left) and output (right) images and quantitative analyses of pgH2AX (A and B), CD3 (C and D), Rag1/Rag2 (E and F), and Dntt (G and H) to assess the differences between WT and Mlh1/ mice in the 4T1 model (n ¼ 4 mice/group). Original magnification, 630. Scale bar, 25 mm. I–L, Comparative images and quantitative analyses of mRNA in situ hybridization for Rag1/Rag2 and IHC for CD3 (I and J) or CD8 (K and L), assessing the percentage of CD3þ or CD8þ cells expressing Rag1/2 in WT and Mlh1/ 4T1 tumors (n ¼ 5 mice/group). Original magnification, 630. Scale bar, 25 mm. M–P, Comparative images and quantitative analyses of mRNA in situ hybridization for Dntt and IHC for CD3 (M and N) or CD8 (O and P) showing the percentage of CD3þ or CD8þ T cells expressing Dntt in WT and Mlh1/ 4T1 tumors (n ¼ 5 mice/group). Original magnification, 630. Scale bar, 25 mm. Statistical analysis: two-tailed unpaired Student t test (B, D, F, H, J, L, N, and P). Mean standard error shown; , P < 0.05; , P < 0.01; , P < 0.001; , P < 0.0001.
Article Snippet: The
Techniques: Expressing, In Situ Hybridization, Two Tailed Test